Chronic lymphocytic leukemia (CLL) is a monoclonal disorder characterized by a progressive proliferation and accumulation of mature yet functionally incompetent lymphocytes. The histologic sample in the image below portrays the appearance of these lymphocytes. CLL is the most common form of leukemia found in adults in Western countries.[1] Some patients die within several years of diagnosis, usually due to complications from CLL, but most patients survive for at least five years.[2]
View Image
Peripheral smear from a patient with chronic lymphocytic leukemia, small lymphocytic variety.
Signs and symptoms
Patients with CLL present with a wide range of signs and symptoms. The onset is insidious, and it is not unusual for CLL to be discovered incidentally when a blood cell count is performed for another reason; 25-50% of patients will be asymptomatic at time of presentation.
Signs and symptoms include the following:
Enlarged lymph nodes, liver, or spleen
Recurrent infections
Loss of appetite or early satiety
Abnormal bruising (late-stage sign)
Fatigue
Night sweats
See Presentation for more detail.
Diagnosis
Patients with CLL typically have a higher-than-normal white blood cell count, which is determined by a complete blood count (CBC). The most recent guidelines from the International Workshop for CLL (iwCLL) state that the diagnosis requires the presence of at least 5000 B-lymphocytes/μL for at least 3 months.[3] The clonality of the B-lymphocytes must be confirmed with flow cytometry. Other tests that may be helpful for diagnosis include bone marrow biopsy and ultrasonography of the liver and spleen. Immunoglobulin testing may be indicated for patients who develop repeated infections.
Staging
Two staging systems are used for CLL: Rai and Binet.
The Rai staging system categorizes patients into low-, intermediate-, and high-risk groups, as follows:
Low risk (formerly stage 0) – Lymphocytosis in the blood and marrow only (25% of presenting population)[4]
Intermediate risk (formerly stages I and II) – Lymphocytosis with enlarged nodes in any site or splenomegaly and/or hepatomegaly (50% of presentations)
High risk (formerly stages III and IV) – Lymphocytosis with disease-related anemia (hemoglobin < 11 g/dL) or thrombocytopenia (platelets < 100 × 109/L) (25% of all patients)
The Binet staging system categorizes patients according to the number of lymph node groups involved. The areas of lymph node involvement considered are the head and neck, including the Waldeyer ring; axillae; groins; a palpable spleen; and a palpable liver.[2]
Stage A – Hemoglobin 10 g/dL or higher, platelets 100 × 109/L or higher, and up to two of the above areas involved.
Stage B – Hemoglobin and platelet levels as in stage A and three or more of the above lymph node areas involved
Stage C – Hemoglobin less than 10 g/dL and/or platelets less than 100 × 109/L
See Workup for more detail.
Management
Patients with early-stage CLL, including low Binet or Rai stages, are not treated with chemotherapy until they become symptomatic or display evidence of rapid progression of disease. Early initiation of chemotherapy has failed to show survival benefit in CLL.[2]
Although combination chemotherapy regimens, including the nucleoside analogue fludarabine, were once the most commonly used first-line therapy in CLL, non-chemotherapy regimens (eg, with Bruton tyrosine kinase [BTK] inhibitors) are currently preferred in most cases. Treatment selection takes into account the molecular and genetic characteristics of the disease and may include the following agents, as monotherapy or in combination[5] :
Acalabrutinib
Obinutuzumab
Venetoclax
Ibrutinib
Zanubrutinib
Rituximab (in combination therapies)
Fludarabine, cyclophosphamide, and rituximab (FCR) was previously a recommended first-line treatment for fit, young patients (< 60 years).[6, 7, 4] However, a 2019 clinical trial called this recommendation into question; it showed superior progression-free and overall survival with ibrutinib-rituximab compared with FCR, in particular in patients without the IGHV mutation, and lower rates of serious infectious complications with ibrutinib-rituximab.[8]
Allogeneic stem cell transplantation is the only known curative therapy for CLL. It should be discussed with patients as a treatment option at the point of first or second relapse.[2]
See Treatment and Medication for more detail.
For patient education information, see Leukemia and Living With Chronic Lymphocytic Leukemia.
The cells of origin in most cases of CLL are clonal B cells arrested in the B-cell differentiation pathway, intermediate between pre-B cells and mature B cells. Morphologically, in the peripheral blood, these cells resemble mature lymphocytes.
CLL B-lymphocytes typically show B-cell surface antigens, as demonstrated by CD19, CD20dim, CD21, and CD23 monoclonal antibodies. In addition, they express CD5, which is more typically found on T cells. Because normal CD5+ B cells are present in the mantle zone of lymphoid follicles, B-cell CLL is most likely a malignancy of a mantle zone–based subpopulation of anergic self-reactive cells devoted to the production of polyreactive natural autoantibodies.
CLL B-lymphocytes express extremely low levels of surface membrane immunoglobulin, most often immunoglobulin M (IgM) or IgM/IgD and IgD. Additionally, they also express extremely low levels of a single immunoglobulin light chain (kappa or lambda).
An abnormal karyotype is observed in the majority of patients with CLL. The most common abnormality is deletion of 13q, which occurs in over 50% of patients. Individuals showing 13q14 abnormalities have a relatively benign disease that usually manifests as stable or slowly progressive isolated lymphocytosis.
Deletion of 11q and 17p as well as trisomy 12 have also been reported, albeit less commonly. The presence of trisomy 12, which is observed in 15% of CLL patients, is associated with atypical morphology and progressive disease. Deletion in the short arm of chromosome 17 has been associated with rapid progression, short remission, and decreased overall survival. 17p13 deletions are associated with loss of function of the tumor suppressor gene p53. Deletions of bands 11q22-q23, observed in 19% of patients, are associated with extensive lymph node involvement, aggressive disease, and shorter survival[2] .
More sensitive techniques have demonstrated abnormalities of chromosome 12. About 40-50% of patients demonstrate no chromosomal abnormalities on conventional cytogenetic studies. However, 80% of patients will have abnormalities detectable by fluorescence in situ hybridization (FISH). Approximately 2-5% of patients with CLL exhibit a T-cell phenotype.
The proto-oncogene Bcl2 is overexpressed in B-cell CLL.[9] The proto-oncogene Bcl2 is a known suppressor of apoptosis (programmed cell death), resulting in a long life for the involved cells. Despite the frequent overexpression of Bcl-2 protein, genetic translocations that are known to result in the overexpression of Bcl2, such as t(14;18), are not found in patients with CLL. Analysis of the molecular pathophysiology of CLL allows the development of therapies that target leukemic cells with these genetic changes. Venetoclax, which is one of the first-line treatment options for CLL, is designed to block the Bcl2 protein.
Studies have shown that this upregulation in Bcl2 is related to deletions of band 13q14. Two genes, named miRNA15a and miRNA16-1, are located at 13q14 and have been shown to encode not for proteins, but rather for a regulatory RNA called microRNA (miRNA).[10, 11] These miRNA genes belong to a family of highly conserved noncoding genes throughout the genome whose transcripts inhibit gene expression by causing degradation of mRNA or by blocking transcription of mRNA.
Deletions of miRNA15a and miRNA16-1 lead to overexpression of Bcl2 through loss of downregulating miRNAs. Genetic analyses have demonstrated deletion or downregulation of these miRNA genes in 70% of CLL cases.[12]
A study of the CLL genome by Wang et al discovered splicing factor 3b (SF3B1) mutations affecting pre-mRNA in 15% of sampled cells.[13] SF3B1 mutations are also found in 20% of myelodysplastic syndrome cases.[14] In future this may provide a therapeutic target.
Investigations have also identified a number of high-risk genetic features and markers, including the following:
Germline immunoglobulin variable heavy chain (IgVH)
IgVHV3-21 gene usage
Increased CD38 expression
Increased Zap70 expression
Elevated serum beta-2-microglobulin levels
Increased serum thymidine kinase activity
Short lymphocyte doubling time (< 6 months)
Increased serum levels of soluble CD23
These features have been associated with rapid progression, short remission, resistance to treatment, and shortened overall survival in patients with CLL.
Germline IgVH has been shown to indicate a poor prognosis. Studies have shown that these patients also have earlier progression of CLL after treatment with chemotherapy. Zeta-associated peptide of 70 kilodaltons (Zap70) is a cytoplasmic tyrosine kinase whose expression has also been associated with a poor prognosis. Cells with germline IgVH often have an increased expression of Zap70; however, studies have shown discordance rates of 10-20% between IgVH mutational status and Zap70 expression levels. Elevated levels of Zap70 are believed to decrease the threshold for signaling through Bcl2, thereby facilitating the antiapoptotic effects of Bcl2.
As is the case with most malignancies, the exact cause of CLL is uncertain. CLL is an acquired disorder, and reports of truly familial cases are exceedingly rare.[15] A meta-analysis of four genome-wide association studies that included 3100 cases of CLL found multiple risk loci. Several of those loci are in close proximity to genes involved in apoptosis, suggesting a plausible underlying biological mechanism.[16]
The American Cancer Society estimates that 18,740 new cases of CLL will be diagnosed in the United States in 2023.[17] The true incidence in the US is unknown and is likely higher, as estimates of CLL incidence come from tumor registries, and many cases are not reported. Although the incidence of CLL has been stable over the last two decades, mortality has been steadily declining.[2]
International Statistics
Although the incidence of CLL in Western countries is similar to that of the United States, CLL is extremely rare in Asian countries (eg, China, Japan), where it is estimated to comprise only 10% of all leukemias. However, underreporting and incomplete registries may significantly underestimate the true incidence of CLL in those countries.
Race-, sex-, and age-related demographics
The incidence of CLL is higher in Whites than in Blacks. The incidence of CLL is higher in males than in females, with a male-to-female ratio of 1.9:1.[2] CLL is a disease that primarily affects the elderly, with the median age at diagnosis being 70 years.[18] In familial CLL (ie, disease in patients with at least one first-degree relative with CLL), median age at diagnosis is 57 years.[19]
The prognosis of patients with CLL varies widely at diagnosis. Some patients die rapidly, within 2-3 years of diagnosis, because of complications from CLL. In most patients CLL initially has a relatively benign course, but eventually enters a progressive, treatment-resistant phase. During this later phase, morbidity is considerable, both from the disease and from complications of therapy.[20, 21]
With the advances in treatment of CLL in recent decades, prolonged survival is possible. However, treatment rarely cures CLL, and mortality rates in patients with CLL remain significantly higher than in the general population.[22]
Prognosis depends on the disease stage at diagnosis as well as the presence or absence of high-risk markers (see Pathophysiology). Given the recent advancements in CLL treatment, the Rai and Binet staging systems do not provide sufficient utility to estimate prognosis. The most accurate prognostic score currently utilized by oncologists is the CLL International Prognostic Index (CLL-IPI), which relies on five independent prognostic factors[2] :
Patient age
Clinical stage (Rai or Binet)
Serum B2 microglobulin level
Mutational status of immunoglobulin heavy chain variable (IGVH)
17p deletion and/or TP53 mutation
Patients are deemed low-, intermediate-, high-, or very high risk depending on the presence or absence of the prognostic factors (see the CLL-IPI calculator). Low-risk patients have over a 90% chance of 5-year overall survival and, therefore, treatment is not recommended. Intermediate-risk patients have a nearly 90% chance of 5-year overall survival and are treated only if they are symptomatic. High-risk patients are managed similarly to intermediate-risk patients, but they have a slightly lower chance of 5-year overall survival at 63%. Very high-risk patients are treated with targeted agents (see Treatment and Medication.
Patients with chronic lymphocytic leukemia (chronic lymphoid leukemia, CLL) present with a wide range of signs and symptoms. The onset is insidious, and it is not unusual for CLL to be discovered incidentally after a blood cell count is performed for another reason. Up to 80% of patients are asymptomatic at the time of diagnosis.[23]
Enlarged lymph nodes are the most common presenting sign in symptomatic patients. A predisposition to repeated infections such as pneumonia, herpes simplex labialis, and herpes zoster may also be seen. Early satiety and/or abdominal discomfort may be related to an enlarged spleen. Mucocutaneous bleeding and/or petechiae may be due to thrombocytopenia. Tiredness and fatigue may be present secondary to anemia; up to 10% of patients with CLL will present with an autoimmune hemolytic anemia.[2]
Richter syndrome or Richter transformation refers to the transformation of CLL into an aggressive large B-cell lymphoma and is seen in approximately 17% of high-risk patients.[2] Patients will often present with a constellation of "B symptoms", including weight loss, fevers, night sweats, and muscle wasting, as well as increasing hepatosplenomegaly and lymphadenopathy. Treatment remains challenging and prognosis is poor, with median survival of a few months.
Physical Examination
In addition to localized or generalized lymphadenopathy, patients may manifest the following:
In patients with CLL, the complete blood count (CBC) with differential shows absolute lymphocytosis, with more than 5000 B-lymphocytes/µL. Lymphocytosis must persist for longer than 3 months. Clonality must be confirmed by flow cytometry. The presence of a cytopenia caused by clonal bone marrow involvement establishes the diagnosis of CLL regardless of the peripheral B-lymphocyte count.
Patients with fewer than 5000 B-lymphocytes/µL with lymphadenopathy and without cytopenias more likely have small lymphocytic lymphoma (SLL), although this diagnosis should be confirmed by lymph node biopsy.
Patients with a clonal B-cell population less than 5000/µL without lymphadenopathy or organomegaly, cytopenia, or other disease-related symptoms have monoclonal B-lymphocytosis (MBL). MBL will progress to CLL at a rate of 1-2% per year[2] .
Microscopic examination of the peripheral blood smear is indicated to confirm lymphocytosis. It usually shows the presence of smudge cells, depicted in the image below, which are artifacts from lymphocytes damaged during the slide preparation.
View Image
Peripheral smear from a patient with chronic lymphocytic leukemia, large lymphocytic variety. Smudge cells are also observed; smudge cells are the art....
Large atypical cells, cleaved cells, and prolymphocytes are also often seen on the peripheral smear and may account for up to 55% of peripheral lymphocytes. If this percentage is exceeded, prolymphocytic leukemia (B-cell PLL) is a more likely diagnosis[2] .
Peripheral blood flow cytometry is the most valuable test to confirm a diagnosis of CLL. It confirms the presence of circulating clonal B-lymphocytes expressing CD5, CD19, CD20(dim), CD23, and an absence of FMC-7 staining. This immunotype differentiates CLL from other hematologic disorders in the differential diagnosis (see DDx).
Consider obtaining serum quantitative immunoglobulin levels in patients developing repeated infections, because monthly intravenous immunoglobulin (IVIG) administration in patients with low levels of immunoglobulin G (IgG), especially levels less than 500 mg/100 mL, may be beneficial in reducing the frequency of infections.
Bone marrow aspiration and biopsy with flow cytometry is not required in all cases of CLL. However, it may be necessary in selected cases to establish the diagnosis and to assess for complications, including cytopenias, which may be explained by concomitant hematologic disorders.
Ultrasonographic studies of the liver and/or spleen may demonstrate hepatomegaly and/or splenomegaly in patients with CLL. Computed tomography (CT) scans of the chest, abdomen, or pelvis are generally not required for staging purposes in CLL. However, imaging can be especially helpful in the assessment of patients with clinical manifestations suggesting obstruction due to lymph node compression of organs or internal structures, such as obstructive uropathy or airway compromise.
Serum free light chain (FLC) assays remain a research tool. Monoclonal and polyclonal abnormalities have been detected in about one-half of CLL cases and appear to be associated with a shorter time from diagnosis to disease progression requiring treatment (time to first treatment; TTFT).[24, 25]
Although not necessary for the diagnosis or staging of CLL, additional molecular testing now exists that may help predict prognosis or clinical course as well as guide treatment choices.[26, 27] The National Comprehensive Cancer Network regards the following tests as informative, but not essential, for determining prognosis, therapy, or both in CLL[5] :
Fluorescence in situ hybridization (FISH) to detect trisomy 12, del(11q), del(13q), del(17p)
TP53 sequencing
Molecular analysis to detect immunoglobulin heavy chain region (IgVH) mutation status
If IgVH testing is not available, determination of CD38 and ZAP-70 expression by flow cytometry, methylation, or immunohistochemistry as surrogate markers
However, a systematic review and meta-analysis by Parikh and colleagues recommended that FISH and IGVH status be performed as standard clinical tests for all patients with newly diagnosed CLL, in those countries with the resources to do so. These authors argue that use of these tests allows the application of powerful prognostic indices to CLL cases.[28]
Favorable prognostic findings include the following[5] :
FISH - Deletion in the long arm of chromosome 13 [del(13q)] as a sole abnormality
TP53 - Wild-type
IgVH - > 2% mutation
Unfavorable prognostic findings include the following[5] :
FISH - Deletion in the short arm of chromosome 17 [del(17p)] or the long arm of chromosome 11 [del(11q)]
TP53 - Mutated
IgVH - ≤2% mutation
Complex karyotype - ≥3 unrelated chromosome abnormalities in more than one cell on karyotype
In addition to a worse prognosis, patients with del(17p) tend to have resistance to therapy with alkylating agents and purine analogues, while those with del(11q) typically have bulky lymphadenopathy at presentation. The poor prognosis seen with del(17p) and del(11q) are independent of the disease stage at presentation. Patients with these abnormalities may benefit from treatment with the monoclonal antibody alemtuzumab, which is a monoclonal antibody against CD52 that has proved effective in patients with high-risk genetic markers such as those noted above.[29, 30, 31, 2] However, none of the poor prognostic markers has been validated as an indication to initiate treatment in asymptomatic patients.[4]
MicroRNA analysis remains a research tool. Visone et al reported that decreases in levels of miR-181b, the most dysregulated microRNA, correlate with progressive disease; a drop of ≥ 50% between sequential samples and/or a baseline miR-181b value ≤ 0.005 differentiate progressive from stable CLL.[32]
Bone marrow aspiration and biopsy with flow cytometry is not required in all cases of CLL, but it may be necessary in selected cases to establish the diagnosis and to assess other complicating features such as anemia and thrombocytopenia. For example, bone marrow examination may be necessary to distinguish between thrombocytopenia due to peripheral destruction and that due to marrow infiltration.
Consider a lymph node biopsy if lymph node(s) begin to enlarge rapidly in a patient with known CLL, to assess the possibility of transformation to a high-grade lymphoma. When such transformation is accompanied by B symptoms, including fever, weight loss, and pain, it is termed Richter syndrome (see Presentation/History and Physical Examination).
Two staging systems are in common use for CLL: the modified Rai (Rai-Sawitsky) staging in the United States and the Binet staging in Europe. Neither is completely satisfactory, and both have often been modified. Because of its historical precedent and wide use, the modified Rai system is described first, followed by the Binet. See also Chronic Lymphocytic Leukemia Staging. These CLL staging systems have been unable to provide information regarding disease progression due to its heterogeneity.
The newest CLL staging system, the International Prognostic Index for Chronic Lymphocytic Leukemia (CLL-IPI), was published in 2016. It is designed to serve as a simple, reliable, and easily applicable method of risk stratification for patients with CLL.[33]
Modified Rai staging
The modified Rai staging system categorizes patients into low-, intermediate-, and high-risk groups, as follows:
Low risk (stage 0 in the original version) – Lymphocytosis in the blood and marrow only (25% of presenting population)[4]
Intermediate risk (formerly stages I and II) – Lymphocytosis with enlarged nodes in any site or splenomegaly and/or hepatomegaly (50% of presentations)
High risk (formerly stages III and IV) – Lymphocytosis with disease-related anemia (hemoglobin < 11 g/dL) or thrombocytopenia (platelets < 100 × 109/L) (25% of all patients)
Binet staging
The Binet staging system categorizes patients according to the number of lymph node groups involved. The areas of lymph node involvement considered are the head and neck, including the Waldeyer ring; axillae; groins; a palpable spleen; and a palpable liver.[2]
Stage A – Hemoglobin 10 g/dL or higher, platelets 100 × 109/L or higher, and up to two of the above areas involved.
Stage B – Hemoglobin and platelet levels as in stage A and three or more of the above lymph node areas involved
Stage C – Hemoglobin less than 10 g/dL and/or platelets less than 100 × 109/L
International Prognostic Index for Chronic Lymphocytic Leukemia
The CLL-IPI is used to determine the prognosis of patients and in turn help in treatment decision making. A weighted gradient is determined using the following 5 factors[33] :
Patient age
Clinical stage (Rai or Binet)
Serum B2 microglobulin level
Mutational status of immunoglobulin heavy chain variable (IGVH)
Deletion 17p (FISH) and/or TP53 mutation
See the CLL-IPI calculator. For treatment considerations based on CLL-IPI risk category, see the table below.
Patients with chronic lymphocytic leukemia (CLL) with a low Binet or Rai stage who are asymptomatic should not be offered treatment. Patients with stable low-stage CLL require only periodic follow-up. In multiple studies and a meta-analysis, early initiation of chemotherapy in such cases has failed to show benefit; indeed, it may increase mortality.[34, 35, 36]
Guidelines from the International Workshop on Chronic Lymphocytic Leukemia (iwCLL) provide clinical and laboratory criteria for initiation of primary, second-line, and subsequent-line treatment.[26] Attempts to consolidate major clinical, chromosomal, and serum markers into a single nomogram/model to guide initiation of treatment for CLL are under way, but as of yet these approaches are far from achieving widespread acceptance.[37]
Treatment is indicated for patients whose CLL progresses or who present with symptomatic disease. The iwCLL guidelines define symptomatic or active disease, which comprise the criteria for starting treatment, by the following[2] :
Weight loss of at least 10% over 6 months
Extreme fatigue (Eastern Cooperative Oncology Group [ECOG] performance status of 2 or more)
Fever of at least 38.0°C for 2 or more weeks with no evidence of infection
Night sweats for at least 1 month with no evidence of infection
Progressive marrow failure (anemia or thrombocytopenia)
Autoimmune anemia or thrombocytopenia not responding to glucocorticoids
Progressive or symptomatic splenomegaly, or massive splenomegaly (at least 6 cm below the costal margin)
Symptomatic or massive lymphadenopathy (at least 10 cm in longest diameter)
Progressive lymphocytosis, as defined by an increase of > 50% in 2 months or a doubling time of less than 6 months
Symptomatic extranodal involvement, including in the spine
A variety of treatment regimens are used in CLL. Although combination chemotherapy regimens including the nucleoside analogue fludarabine were once the most commonly used first-line therapies, non-chemotherapy regimens are currently preferred in the majority of cases. Recommended biologic agents for first-line therapy include the Bruton tyrosine kinase (BTK) inhibitors, a B-cell lymphoma inhibitor (venetoclax), and monoclonal antibodies. Allogeneic stem cell transplantation is the only known curative therapy. Complete response (CR) is defined by absence of lymphocytosis, lymphadenopathy, and organomegaly, without significant cytopenias.
Treatment selection takes into account the molecular and genetic characteristics of the disease and may comprise monotherapy or combination therapy. Different regimens are recommended for those with a del(17p) or tp53 mutation versus those without those mutations.[5] In addition, different regimens are often recommended for those older than 65 years, or younger than age 65 but with significant comorbidities, versus fit patients who are under 65 years of age.[5] Fludarabine, cyclophosphamide, and rituximab (FCR) remains a recommended first-line treatment only for fit young patients with IGVH hypermutation, as long-term remissions have been reported in this setting.[6, 7, 4]
See also Chronic Lymphocytic Leukemia Treatment Protocols.
Biologic agents entered clinical practice for second-line treatment of CLL but have also become preferred agents for first-line therapy. Currently recommended non-chemotherapy regimens for CLL include monotherapy and combination therapy with agents such as Bruton tyrosine kinase (BTK) inhibitors, monoclonal antibodies, and a B-cell lymphoma inhibitor, among others.[5]
Cytostatic Agents
Monotherapy with the alkylating chemotherapy agents, including chlorambucil, had been the first-line therapy for several years. Chlorambucil offers the advantages of oral administration, low cost, and limited toxicity. However, it has an extremely low complete response (CR) rate and is therefore used only for palliative purposes in elderly or debilitated patients.[6, 7]
Bendamustine is an alkylating agent that is administered by intravenous infusion. A phase III trial comparing bendamustine with chlorambucil in treatment-naive patients who were not deemed candidates for more aggressive regimens, such as fludarabine, cyclophosphamide, and rituximab (FCR), showed no difference in overall survival, but complete response was higher with bendamustine than with chlorambucil (21% vs 10%) and progression-free survival (PFS) was longer (21 months vs 9 months). Bendamustine use did not compromise quality of life but did produce greater toxicity.[38] In a German phase II study of bendamustine combined with rituximab in 72 pretreated patients, the overall response rate (ORR) was 59% and PFS was almost 15 months.[39]
The current focus in CLL is on targeted therapies in various combinations, which are discussed below. These have already been proven superior to chemoimmunotherapy. Moreover, the risk of secondary malignancies (eg, myelodysplastic syndromes, acute myeloid leukemia) with chemoimmunotherapy further strengthens the argument for targeted therapy.[40]
Bruton tyrosine kinase inhibitors
Bruton tyrosine kinase (BTK) leads to downstream activation of B-cell survival pathways, including nuclear factor-kB (NF-kB), which helps prevent apoptosis and promotes survival of B-cells. BTK inhibitors block this pathway and therefore promote destruction of the leukemic cells. The first-generation BTK inhibitor ibrutinib and the second-generation inhibitors acalabrutinib and zanubrutinib are approved for both first-line and subsequent therapy of CLL.
Second-generation BTK inibitors have proved successful for treatment of CLL in patients with ibrutinib intolerance, but have not been effective in CLL refractory to ibrutinib. However, third-generation BTK inhibitors, currently under investigation, are showing promise in overcoming BTK inhibitor resistance.[41]
Ibrutinib
In 2014, the FDA approved the BTK inhibitor ibrutinib (Imbruvica) for CLL in patients who had received at least one previous therapy. Accelerated approval was based on a clinical study of 48 previously treated participants. On average, participants were diagnosed with CLL 6.7 years prior to the study and had received four previous therapies. All study participants received oral ibrutinib 420 mg/day until unacceptable toxicity or disease progression. The overall response rate was nearly 58% and the estimated PFS at 26 months was 75%. At the time of the study, the duration of response ranged from 5.6 to 24.2 months. An improvement in survival or disease-related symptoms has not been established.[42]
Also in 2014, the FDA approved an expanded indication for ibrutinib for the treatment of CLL patients with a deletion in chromosome 17, which is associated with poor response to standard treatments.[43] Approval of the expanded indication was based on an analysis of a subgroup of 127 CLL patients with 17p deletion from the RESONATE study, in which patients treated with ibrutinib experienced a 75% reduction in the risk of disease progression or death.[43, 44]
In 2016, the FDA further expanded the indication for ibrutinib to include treatment-naïve patients. Approval was based on the RESONATE-2 study (n=269) that compared ibrutinib with chlorambucil in treatment-naïve patients with CLL who were 65 years of age or older. During a median follow-up of 18.4 months, PFS was significantly longer with ibrutinib than with chlorambucil (median, not reached vs 18.9 mo), with risk of progression or death 84% lower with ibrutinib than with chlorambucil (P< 0.001). Ibrutinib significantly prolonged overall survival (estimated 98% vs 85%); relative risk of death was 84% lower in the ibrutinib group than in the chlorambucil group (P=0.001).[45]
The CLL12 trial, published in 2022, showed that ibrutinib prolonged survival in Binet stage A patients who had not received prior treatment for CLL. Statistically, ibrutinib did not increase overall toxicity compared with no treatment.[46] However, the clinical recommendation for first-line therapy in early-stage CLL remains to "watch and wait".[47]
Unfortunately, hypertension has proved to be an extremely common adverse event in patients taking ibrutinib. In a study of 562 consecutive patients treated with ibrutinib for B-cell malignancies, Dickerson et al found that new hypertension developed in 71.6% of patients and existing hypertension worsened in an additional 6.7% of patients over a median of 30 months. Of the new hypertension cases, 17.7% were high-grade (blood pressure > 160/100 mm Hg). New or worsened hypertension was associated with increased risk of major adverse cardiovascular events (hazard ratio 2.17).[48]
Other severe and occasionally fatal cardiac events that may occur in patients taking ibrutinib include supraventricular and ventricular arrhythmias, heart failure, and conduction disorders.[49] Development of atrial fibrillation (AF), particularly in elderly patients, typically necessitates starting anticoagulation, which also increases bleeding risk.[2]
Resistance to ibrutinib has been reported, and the mechanisms of resistance are only partially understood. It is frequently due to BTK mutations at the ibrutinib binding site that interfere with the binding of ibrutinib, or to gain-of-function mutations in PLCG2 that cause the pathway to be activated without BTK interaction. Whole-exome sequencing in patients with resistance identified a cysteine-to-serine mutation in BTK at the binding site. In some cases, both mutations occur together. Some initial studies have also suggested that BTK inhibitor therapy can lead to clonal selection and expansion.[50] [51] Patients who experienced relapse of CLL were significantly more likely to have acquired a mutation of BTK or PLGC2.[2] Analysis for potential mutations may also serve as a guide to further targeted interventions for these patients prior to relapse.
A retrospective population-based cohort study of patients with CLL treated with ibrutinib demonstrated an increased risk of death in patients concomitantly receiving a CYP3A inducer; death in those cases was most commonly due to CLL progression. Although the mechanisms are not completely understood, this phenomenon may be partly attributed to lower levels of ibrutinib in patients exposed to CYP3A inducers.[52]
Acalabrutinib
In 2019, the FDA approved the BTK inhibitor acalabrutinib (Calquence) for treatment of adults with CLL and SLL. Acalabrutinib is an irreversible BTK inhibitor and is more selective than ibrutinib.
Approval was based on two randomized controlled trials in patients with CLL: ELEVATE-TN and ASCEND. In ELEVATE-TN, which included 535 patients with previously untreated CLL, PFS was significantly longer with acalabrutinib as monotherapy or in combination with obinutuzumab, as compared with obinutuzumab plus chlorambucil. In a 4-year follow-up of ELEVATE-TN, the efficacy and safety of acalabrutinib plus obinutuzumab and acalabrutinib monotherapy were maintained, with low rates of treatment discontinuation.[53] In ASCEND, which included 310 patients with relapsed or refractory CLL after at least one prior systemic therapy, PFS was significantly longer with acalabrutinib than with idelalisib or bendamustine plus a rituximab product.[54]
Another study compared acalabrutinib 200 mg once daily versus 100 mg twice daily in 99 patients with treatment-naive CLL. The ORR was found to be 97%. The twice-daily dosing group showed better trough BTK occupancy levels, suggesting that twice-daily dosing may be more favorable.[55]
However, resistance to acalabrutinib has been reported, with genetic mutations that are similar to those reported with ibrutinib.[2] Moreover, in a study comparing first-line CLL therapy with alcalabrutinib versus ibrutinib, patients receiving acalabrutinib monotherapy were 89% more likely to start a next-line treatment than patients treated with ibrutinib. This study used time to next treatment as a clinically meaningful surrogate measure for disease progression.[56]
Adverse effects of acalabrutinib include diarrhea, headaches, neutropenia, thrombocytopenia, and pneumonia. Hypertension and AF have also been reported, but less commonly than with ibrutinib, suggesting that acalabrutinib may be tried in patients with a history of intolerance to ibrutinib. Although ibrutinib and acalabrutinib have comparable efficacy and rates of PFS, acalabrutinib is typically preferred due to its more favorable adverse effect profile, particularly with regard to cardiovascular effects.[2]
Zanubrutinib
Zanubrutinib is a second-generation, irreversible BTK inhibitor with higher specificity than ibrutinib. In January 2023, the FDA granted accelerated approval for zanubrutinib to treat patients with CLL or SLL.[57]
Approval was based on the phase III SEQUOIA and ALPINE trials. In SEQUOIA, 479 treatment-naïve patients received either zanubrutinib until disease progression/unacceptable toxicity or bendamustine plus rituximab for 6 cycles. Median PFS was not reached in the zanubrutinib arm and was 33.7 months in the bendamustine plus rituximab arm. In a separate, nonrandomized SEQUOIA cohort of 110 patients with a 17p deletion who received zanubrutinib, the overall response rate (ORR) was 88% and the median duration of response (DoR) was not reached over the 25-month follow-up period.[57]
In ALPINE, which compared zanubrutinib with ibrutinib in 652 patients with relapsed or refractory CLL/SLL, the ORR was 80% in the zanubrutinib arm vs 73% in the ibrutinib arm. Like the SEQUOIA cohort, the median DoR was not reached in either arm over the 14-month follow-up period. Median PFS was not reached in the zanubrutinib arm and was 35 months in the ibrutinib group.[57]
Common adverse effects of zanubrutinib include petechiae and contusions. Cardiovascular effects, including AF, are less frequent with zanubrutinib than with ibrutinib and its overall toxicity profile is more favorable.[2, 58]
Pirtobrutinib
Pirtobrutinib is a highly selective and reversible BTK inhibitor that received accelerated approval for use in mantle cell lymphoma in early 2023. Accelerated approval for use in CLL/SLL was granted in December 2023. In a clinical trial published in 2021, 323 patients with a history of treated B-cell malignancies, including 121 patients with CLL/SLL, were treated with pirtobrutinib across seven dose levels. The ORR was found to be 62%, and the ORR was similar even in patients with a history of BTK inhibitor resistance.[59]
A second study corroborated these results. Patients with CLL or SLL received pirtobrutinib (N=317), including 247 who had previously received a BTK inhibitor (a median 3 previous lines of therapy; range, 1 to 11), and 100 patients (40.5%) had also received a B-cell lymphoma 2 (BCL2) inhibitor (eg, venetoclax). ORR to pirtobrutinib was 73.3% (82.2% when partial response with lymphocytosis was included). The median PFS was 19.6 months.[60]
The most commonly reported adverse effects were infections, bleeding, diarrhea, fatigue, and neutropenia. Of note, cardiac arrhythmias, including atrial fibrillation and atrial flutter, were infrequently observed, and the vast majority of patients in the study tolerated the medication well; only 1% discontinued treatment because of a treatment-related adverse event.[59, 60]
B-cell lymphoma inhibitors
Venetoclax
Venetoclax (Venclexta) is a BH3 mimetic that is a selective inhibitor of the B-cell lymphoma 2 (Bcl-2) regulator protein, an antiapoptotic protein.[61] It was approved by the FDA in 2016 for patients with CLL with 17p deletion, as detected by an FDA-approved test, who have received at least one prior therapy. Venetoclax is typically given weekly in a dose ramp-up regimen over 4-5 weeks until the maximum dose of 400 mg is reached. Patients take 400 mg daily indefinitely, until disease progression or adverse effects occur.[2]
In 2019, the FDA approved the combination of venetoclax and obinutuzumab for use in patients with previously untreated CLL.[62] The combination needs to be taken for only 1 year; in contrast, standard regimens need to be continued longer, or even indefinitely.[63]
In a randomized, open-label, phase III trial in 389 patients with relapsed or refractory CLL, treatment with venetoclax plus rituximab resulted in 2-year PFS of 84.9%, compared with 36.3% in patients treated with bendamustine plus rituximab (hazard ratio for progression or death, 0.17; 95% confidence interval [CI], 0.11 to 0.25; P< 0.001). The benefit of venetoclax-rituximab was maintained across all clinical and biologic subgroups, including patients with and without chromosome 17p deletion.[64] Study patients received venetoclax for up to 2 years, plus rituximab for the first 6 months; or bendamustine plus rituximab for 6 months. In a post-treatment study, on median follow-up of 36 months, PFS and overall survival remained superior with venetoclax-rituximab, demonstrating the feasibility of this fixed-duration regimen.[65]
A phase II study of the combination of ibrutinib and venetoclax in 80 previously untreated high-risk and older patients with CLL reported that after 12 cycles, 88% of patients had complete remission or complete remission with incomplete count recovery, and 61% of patients had remission with undetectable minimal residual disease (MRD).[66] In the phase III GLOW trial, ibrutinib-venetoclax demonstrated significantly higher PFS and more sustained treatment responses as first-line CLL treatment in older patients, particularly in those with comorbidities, compared with chlorambucil-obinutuzumab.[67] However, there is still insufficient evidence to recommend ibrutinib-venetoclax as a standard first-line therapy in the elderly. Adverse effects include neutropenia, diarrhea, and hypertension.
A phase II trial reports promising results with a three-drug combination of venetoclax, acalabrutinib, and obinutuzumab, with 83% of patients showing undetectable MRD at a median follow-up of 35 months. The trial involved 68 patients with previously untreated high-risk CLL.[68]
Resistance to venetoclax may occur through Richter transformation to diffuse large B-cell lymphoma or recurrence of CLL. Richter transformation has been associated with BCL2 mutations that prevent the binding of venetoclax to BCL2 domains; BTG1 mutations; upregulation of clones that express a convoluted kayotype; mutations in cyclin-dependent kinase genes; and the enhanced expression of BCL-XL and MCL1, which are anti-apoptotic.[50]
Monoclonal antibodies
The following monoclonal antibodies are approved for use in CLL:
Obinutuzumab
Ofatumumab
Alemtuzumab
Other monoclonal antibodies in development that are undergoing study in CLL include hLL1, epratuzumab, and lumiliximab. Despite promising results in a phase I/II trial of lumiliximab, a phase III trial comparing FCR with and without lumiliximab in patients with relapsed CLL was stopped early when an interim analysis failed to show sufficient efficacy of the FCR-lumiliximab combination.[69]
Obinutuzumab
Obinutuzumab (Gazyva) is a CD20-directed cytolytic monoclonal antibody. It was approved by the FDA in 2013 for previously untreated CLL in combination with chlorambucil. Obinutuzumab is the first drug with a breakthrough therapy designation to receive FDA approval. This designation means that obinutuzumab has the potential to offer a substantial improvement over available therapies for patients with serious or life-threatening diseases.
Approval of obinutuzumab was based on a pivotal phase III trial in 356 patients (mean age, 73 y) with previously untreated CLL, in which median PFS was significantly better in patients who received obinutuzumab in combination with chlorambucil than in those treated with chlorambucil alone (23 vs 11.1 mo; P< 0.0001).[70] Those results effectively ended the use of chlorambucil as monotherapy.[71]
In 2019, the FDA approved the use of obinutuzumab in combination with ibrutinib for previously untreated CLL or SLL. This was the first approval for a non-chemotherapy combination regimen for treatment-naïve patients with CLL/SLL.The approval was supported by the phase III iLLUMINATE trial, in which ibrutinib plus obinutuzumab proved superior to chlorambucil plus obinutuzumab.[72] The GAUGUIN trial also demonstrated the efficacy of obinutuzumab in CLL, with a median PFS of 10.7 months.[2]
Ofatumumab
Ofatumumab (Arzerra), an anti-CD20 monoclonal antibody, was approved by the FDA in 2010 for CLL that is refractory to fludarabine and alemtuzumab.[73] It has an increased binding affinity to CD20 and greater complement-dependent cytotoxicity relative to ritixumab, making it more favorable for cells expressing low levels of CD20.[2] Ofatumumab is also approved for use in combination with chlorambucil for untreated patients with CLL in whom fludarabine-based therapy is inappropriate.[74, 75]
In 2016, ofatumumab’s indication was expanded to include extended treatment as a single agent for patients who are in complete or partial response after at least two lines of therapy for recurrent or progressive CLL.[76] Also in 2016, ofatumumab’s indication was further expanded to include use in combination with fludarabine and cyclophosphamide in relapsed CLL. Approval was based on the COMPLEMENT 2 international trial (N = 365) in patients with relapsed CLL, in which the addition of ofatumumab to fludarabine and cyclophosphamide was associated with improved PFS.[77] However, for commercial reasons, the manufacturer withdrew obatumumab from non-US markets in 2018, limiting its availability outside the United States to compassionate use programs for patients currently benefiting from the drug.[78] Ofatumumab is now primarily used for multiple sclerosis.
Alemtuzumab
Alemtuzumab is a monoclonal antibody directed at CD52. Alemtuzumab has been shown to be effective in treating CLL with high-risk genetic markers, including the p53 mutations [del(17p13.1)]. This is in contrast to rituximab, which is not effective in p53 mutation–bearing CLL. Although very effective in clearing the bone marrow of disease, alemtuzumab has shown only limited activity in clearing bulky lymphadenopathy. However, alemtuzumab appears to have a role in consolidation therapy for the elimination of MRD.[30, 31, 79, 80] In one study, 38% of patients treated with alemtuzumab consolidation after induction chemotherapy had molecular disease remission. Of note, three patients in this study developed Epstein-Barr virus–positive large B-cell lymphoma; two of these lymphomas resolved spontaneously and the third responded to cidofovir and immunoglobulin.
Two phase II studies have evaluated aggressive treatment with FCR and alemtuzumab for high-risk CLL as frontline[81] and salvage treatment.[82] Although the median PFS was 38 months and the median overall survival was not reached in the frontline study, this regimen may be of interest as means of achieving complete response in the 17p deletion CLL population before allogeneic stem cell transplantation, in selected patients with excellent performance status.
In pretreated patients, when used as salvage and compared with FCR, the addition of alemtuzumab to FCR did not improve PFS or overall survival. Serious infections developed in 74% of patients at some point during or after treatment.[82] The German CLL Study Group prematurely closed a phase III trial involving alemtuzumab consolidation due to severe infections in the alemtuzumab arm; however, this has not been seen in other studies to date.
Antiviral prophylaxis and prophylactic antibiotics for Pneumocystis jiroveci are recommended for patients receiving alemtuzumab during and for 2-4 months after treatment, or until their CD4 count exceeds 250 × 109 cells. Cytomegalovirus (CMV) polymerase chain reaction (PCR) testing is also recommended to monitor for CMV reactivation. If CMV is detected, alemtuzumab should be discontinued, and appropriate treatment initiated until CMV becomes undetectable.
A 2012 Cochrane Database review including five randomized controlled trials (845 patients) failed to show improvement in survival or PFS when alemtuzumab was compared with rituximab (two trials). PFS (but not overall survival) was improved when alemtuzumab was compared with chlorambucil, at the price of increased CMV infections in the alemtuzumab arm (one trial). In two other trials reviewed, alemtuzumab was superior to no therapy for overall survival in one trial but one of the two trials had to be closed prematurely due to severe infections in the alemtuzumab group.[83] The findings support continued recommendation of alemtuzumab only for those patients with p53 mutation (p17) and those in whom a fludarabine-based regimen has failed, and not as a first-line agent due to the high rate of infections and other serious adverse events.
Of note, the license for alemtuzumab was withdrawn in 2012, but it has continued to be available internationally through compassionate programs and is being used to treat multiple sclerosis. However, its use in CLL has been progressively less prevalent, given the introduction of targeted treatment regimens.
Combination therapy with monoclonal antibodies
Rituximab as a single agent has produced only partial responses of short duration, but it has been used extensively in combination with other biologic agents (ibrutinib, venetoclax, lenalidomide) and chemotherapy drugs (eg, fludarabine). Patients with trisomy 12q may express higher levels of CD20, thus making tumor cells more vulnerable to biologics against CD20.[84]
Fludarabine has been shown to downregulate CD55 and CD59; these are proteins involved in complement resistance, and their loss enhances the action of rituximab. Fludarabine combined with rituximab has produced higher clinical remission rates than fludarabine alone in clinical trials. In a prospective, single-arm study of patients treated initially with fludarabine and rituximab, median overall survival was 85 months. After 5 years, 71% of patients were alive and 27% remained free of disease.[85]
The combination of fludarabine and cyclophosphamide with rituximab (FCR) has shown to produce better clinical response rates than those seen with either fludarabine or fludarabine and cyclophosphamide (FC) in salvage therapy for patients with previously treated CLL. A study by Robak et al in 552 patients with previously treated CLL found that after a median follow-up of 25 months, median PFS was 30.6 months with FCR versus 20.6 months with FC. In addition, patients receiving FCR demonstrated significantly better event-free survival, response rate, complete response rate, duration of response, and time to new CLL treatment or death.[86]
A study in treatment-naive patients with CD20-positive CLL found that 3 years after randomization, PFS was 65% with FCR versus 45% with FC. Overall survival was 87% versus 83%, respectively. Grade 3 and 4 neutropenia and leukocytopenia were more common with FCR, but other adverse effects, including severe infections, were not increased.[87]
A phase III study in patients with previously treated CLL found that fludarabine plus alemtuzumab (n=168) resulted in longer PFS than did fludarabine alone (n=167; median 23.7 months versus 16.5 months, P=0·0003). Overall survival was also superior with the combination.[88] However, a study of the combination of cyclophosphamide, fludarabine, alemtuzumab, and rituximab (CFAR) as salvage therapy for heavily pretreated patients with CLL found that although CFAR produced good response rates, there was no benefit in survival outcomes and CFAR was associated with a high rate of infectious complications.[89]
The combination of obinutuzumab with chlorambucil was found to be superior to rituximab/chlorambucil in a study of 781 treatment-naive CLL patients.[90] Median PFS was 27 months in the obinutuzumab/chlorambucil group, compared with 15 months in the rituximab/chlorambucil group; the ORRs were 78% vs 65%, respectively. At the end of treatment, MRD in bone marrow was 19.5% in patients who received obinutuzumab/chlorambucil, versus 2.6% in patients who received rituximab/chlorambucil, and MRD in blood was 37.7% and 3.3%, respectively.[90]
Phosphoinositide 3-kinase inhibitors
Phosphatidylinositol 3-kinase (PI3K) is one of the tyrosine kinases that play a role in B-cell receptor (BCR) signaling.[2] The PI3K inhibitors idelalisib and duvelisib are approved for treatment of relapsed CLL, but have high rates of adverse effects (ie, infections and autoimmune complications). Thus, these agents are typically used only if the less toxic therapies are no longer effective.
Idelalisib
In 2014, the FDA approved the oral kinase inhibitor idelalisib (Zydelig) for the treatment of relapsed CLL, relapsed follicular B-cell non-Hodgkin lymphoma, and relapsed SLL.[91, 92] Idelalisib works by promoting apoptosis in the CLL cells without affecting alternative immune pathways, including T-cells and natural killer cells.[2] The drug was approved for the relapsed CLL indication for use in combination with rituximab. Approval was based on a placebo-controlled study in 220 patients in which patients treated with idelalisib plus rituximab showed significantly longer PFS than those who received placebo plus rituximab (10.7 vs 5.5 months, respectively).[75] It has also proved effective in patients with bulky lymphadenopathy or treatment-refractory disease. The most commonly observed adverse effects include pneumonia, neutropenic fever, and diarrhea.[2]
Duvelisib
Duvelisib (Copiktra) was approved in 2018 for relapsed or refractory CLL or SLL in patients who had received at least two prior therapies. Approval was based in part on the phase III DUO clinical trial (n=319), in which duvelisib reduced the risk of disease progression or death by 48% compared with ofatumumab, and more patients responded to duvelisib than to ofatumumab (73.8% vs 45.3%; P < 0.0001 for both CLL and SLL). The median PFS was longer in those treated with duvelisib (13.3 vs 9.9 months; P < 0.0001), including in patients with the del(17p) mutation (12.7 vs 9.0 months; P = 0.0011). Overall survival (OS) was similar between the 2 treatment cohorts (P = 0.48)[93] . The most commonly observed adverse effects include hematologic toxicities, transaminitis, diarrhea, and opportunistic infections.[2]
Umbralisib
Umbralisib is a dual inhibitor of PI3K and casein kinase 1 (CK1) epsilon.[2] The UNITY-CLL study, which analyzed umbralisib in combination with ublituximab (the U2 regimen) demonstrated superior PFS versus standard of care chemoimmunotherapy in patients with treatment-naive and refractory CLL.[94] However, updated findings from the UNITY-CLL trial continued to show a possible increased risk of death in patients receiving umbralisib, and the FDA determined that the risks of treatment with umbralisib outweigh its benefits. Consequently, the drug’s manufacturer voluntarily withdrew umbralisib from the market for the approved uses in marginal zone lymphoma and follicular lymphoma.[95]
Lenalidomide
Lenalidomide is an immunomodulatory drug approved for use in multiple myeloma, myelodysplastic syndrome with deletion of chromosome 5q, and certain lymphomas. In phase II trials, lenalidomide demonstrated efficacy in CLL that is relapsed or refractory to previous treatments that included fludarabine, although better results were reported with first-line use of lenalidomide.[96] In 2013, however, the FDA halted a clinical trial of single-agent lenalidomide for the first-line treatment of CLL due to increased risk of death as compared with single-agent chlorambucil. Lenalidomide should not be used for treatment of CLL outside of a clinical trial.[97]
Chimeric antigen receptor (CAR) T-cell therapy
CAR T-cell therapy is a novel treatment in which the patient's T-cells are collected from a blood sample and transformed in the laboratory so that they will recognize a specific antigen (eg, CD19) on the surface of cancer cells, attack the cancer cells, and proliferate in vivo. The CAR T-cells are then grown in large quanitity and infused into the patient.[98] For more information, see Cancer Immunotherapy with Chimeric Antigen Receptor (CAR) T-Cells.
Due to weak response levels and brief remission periods, clinical developments focusing on individual CAR T-cell monotherapy have slowed down.[50] However, Gauthier et al reported encouraging success with CAR T-cell therapy combined with ibrutinib in 19 patients with ibrutinib-refractory CLL. In the trial, 61% of patients achieved marrow remission, with undetectable MRD; in that subset, the 1-year overall survival probability was 86%, and PFS probabilitiy was 59%. The treatment was well tolerated, with low severity of cytokine release syndrome.[99]
Full remission of CLL has been reported in approximately a quarter of patients treated with CAR T-cell therapy. In 2022, Melenhorst et al reported decade-long remission of CLL in two patients who had achieved complete remission with CAR T-cell therapy in 2010. The patients continued to show detectable levels of CAR T-cells, but the cell population had evolved from consisting primarily of killer T-cells to consisting primarily—in one of the patients, exclusively—of proliferative CD4-positive CAR T-cells, with continuing capability of destroying leukemia cells.[100]
Treatment in elderly patients
While chlorambucil is a neglected drug in the United States, due to very low rates of objective response and inferior results compared with newer biologic agents, in Europe it is still used as a first-line agent in elderly, fragile populations, which make up the bulk of true CLL cases. In the CLL5 study comparing fludarabine with chlorambucil (median age of 70 years), while there was a significantly higher response rate with fludarabine, PFS was similar (19 vs 18 months). Overall survival was not significantly affected either, although it was 46 months with fludarabine compared with 64 months for chlorambucil.[101]
Prospective and retrospective studies have shown that chlorambucil plus rituximab is safe and effective in older patients with CLL.[102, 103] For example, in the retrospective GIMEMA study, which used that combination as front-line treatment in 102 elderly (≥65 years) and/or unfit patients with CLL, estimated survival rates were 86.1% (95% CI 79.4–93.5%) after 48 months and 81.2% (95% CI 72.4–91.2%) after 60 months.[102]
Ibrutinib is approved for first-line treatment in elderly patients. Approval was based on the RESONATE-2 study (n=269), which compared ibrutinib with chlorambucil in treatment-naïve patients with CLL who were aged 65 years or older. Statistically significant improvement in PFS and objective response rate was observed with ibrutinib compared with chlorambucil.[45]
Allogeneic stem cell transplantation is the only known curative therapy for CLL. The optimal timing of transplantation is still being investigated[104] ; however, delay of transplantation until development of refractory disease is known to result in worse outcomes. Nevertheless, most patients are elderly and too fragile for upfront stem-cell transplantation to be considered in their first clinical remission. Thus, the targeted and biologic agents remain the first-line treatment in patients who meet criteria for treatment.
The effectiveness of non-myeloablative transplantation has shown that there is a graft-versus-leukemia effect in CLL. Autologous transplantation after high-dose conditioning has not been shown to provide a survival advantage and is not recommended outside the setting of a clinical trial. A study by Michallet et alindicated that patients who had responded to first-line or second-line therapy were more likely to experience 5-year event-free survival (EFS) if they underwent autologous stem cell transplantation instead of observation (5-year EFS rates were 42% vs 24%, respectively); however, transplantation had no effect on overall survival.[105]
Alemtuzumab is being investigated for use in hematopoietic stem cell transplantation (HSCT). This agent may play an important role in the elimination of MRD in patients undergoing autologous transplantation, while, at the same time, the lack of CD52 on hematopoietic stem cells prevents interference with stem cell collection. The addition of alemtuzumab to non-myeloablative conditioning regimens for allogeneic HSCT appears to decrease the incidence of graft versus host disease (GVHD), but it may be associated with increased rates of cytomegalovirus reactivation.
Autoimmune manifestations in CLL are myriad, as follows[106] :
Positive direct antiglobulin test (DAT; Coombs test) without autoimmune hemolytic anemia (AIHA)
AIHA
Immune thrombocytopenia (ITP)
Pure red cell aplasia
Autoimmune neutropenia
Cold agglutinin disease
Paraneoplastic pemphigus
Neuropathies
Evans syndrome
Up to 25% of patients with CLL demonstrate autoimmune anemia, thrombocytopenia, or both. Simultaneously, immune incompetence is present, characterized by a progressive profound hypogammaglobulinemia, predisposing patients to a number of infections, especially bacterial pneumonias, as well as sepsis and meningitis. Common pathogens include Streptococcus pneumoniae, Staphylococcus aureus, and Haemophilus influenzae. Viral infections, such as from herpes zoster and herpes simplex, may also occur. Infections account for nearly half of all deaths in patients with CLL.
Yearly influenza and pneumococcal vaccination every 5 years is indicated; however, live vaccines should not be given to patients with CLL. Growth factors may be used to decrease the duration of neutropenia following chemotherapy. Patients experiencing frequent bacterial infections associated with hypogammaglobulinemia are likely to benefit from monthly infusions of intravenous immunoglobulin (IVIG). Studies of prophylactic IVIG in patients with CLL have not demonstrated a survival benefit, but have shown a significant decrease in the occurrence of major infections and a significant reduction in clinically documented infections.[107]
Prednisone alone, usually in a dose of 20-60 mg daily initially, with subsequent gradual dose reduction, may be useful in patients with autoimmune hemolytic anemia (AIHA). Rituximab, alone or as part of a combination regimen, can be very effective in eliminating the B-cell clone that induces autoimmune disorders, particularly for patients with autoimmune thrombocytopenia. IVIG can be used as a short-term measure in patients who have severe thrombocytopenias or in patients anticipating surgery. Thrombopoietin receptor agonists have been used with some success, as in primary immune thrombocytopenia.[108] The previous notion that purine analogs such as fludarabine are more prone to result in autoimmune cytopenias has been challenged by data from studies such as the Leukemia Research Foundation Chronic Lymphocytic Leukaemia Trial 4 (LRF CLL4).[109]
Occasionally, nonimmune manifestations due to antibodies may occur in CLL. For example, glomerulonephritis due to monoclonal gammopathy has been reported in patients with CLL.[110, 111]
Extremely high white blood cell counts (> 300,000/µL) may produce a hyperviscosity syndrome with altered central nervous system function and/or respiratory insufficiency. Leukocytapheresis and urgent therapy with prednisone and chemotherapy may be required. Virtually all patients requiring therapy should also be given allopurinol to prevent uric acid nephropathy and should be monitored for development of tumor lysis syndrome.
Refractory splenomegaly and pancytopenia are not uncommon in patients with advanced CLL. Occasionally, these patients require splenectomy. Substantial improvements in hemoglobin and platelet counts are observed in up to 90% of patients undergoing splenectomy. All patients with CLL who are to undergo splenectomy should be immunized at least 1 week in advance against pneumococcus, Haemophilus influenzae, and Neisseria meningitidis.
Guidelines for the diagnosis and treatment of chronic lymphocytic leukemia (CLL) have been issued by the following organizations:
National Comprehensive Cancer Network (NCCN)[5]
International Workshop on Chronic Lymphocytic Leukemia (iwCLL)[26]
European Society for Medical Oncology (ESMO)[112]
Diagnosis and Staging
All three guidelines are in agreement that the diagnosis of CLL requires the presence of ≥5 × 109/L B-lymphocytes in the peripheral blood and the clonality of the circulating B lymphocytes should be confirmed by flow cytometry. The iwCLL guidelines also require persistence of lymphocytosis for longer than 3 months, while NCCN and ESMO do not.[5, 26, 112]
For adequate immunophenotyping to establish diagnosis by flow cytometry, the NCCN recommends using the cell surface markers kappa/lambda, CD19, CD20, CD5, CD23, and CD10. If flow cytometry is used to establish the diagnosis, cytospin for cyclin D1 or fluorescence in situ hybridization (FISH) for t(11;14); t(11q;v) should also be included.[5]
The NCCN recommends proceeding with lymph node biopsy if the diagnosis is not established by flow cytometry. Diagnosis on lymph node biopsy requires immunophenotyping of CD3, CD5, CD10, CD20, CD23, and cyclin D1.[5]
ESMO guidelines note that leukemia cells are characteristically small, mature-appearing lymphocytes with a narrow border of cytoplasm and a dense nucleus lacking discernible nucleoli, and having partially aggregated chromatin. Larger, atypical lymphocytes or prolymphocytes cannot exceed 55%.[112]
The iwCLL guidelines also allow for the diagnosis of CLL in the presence of a cytopenia caused by clonal bone marrow involvement regardless of the peripheral B-lymphocyte count.[26]
Additional tests recommended prior to initiation of treatment include:
Fluorescence in situ hybridization (FISH) to detect cytogenetic abnormalities, in particular a deletion of chromosome 17 [del(17p)], 13[del(13q)] and 11 [del(11q)] and trisomy of chromosome12[5, 26, 112]
Molecular analysis to detect IGHV mutation status[26, 5]
Determination of CD38 and ZAP-70 expression by flow cytometry or immunohistochemistry[5]
TP53 sequencing[5]
Prior to chemoimmunotherapy, alemtuzumab or allogeneic stem cell transplantation, testing for hepatitis B and C viruses, cytomegalovirus (CMV), and human immunodeficiency virus (HIV)[26, 112]
Unilateral bone marrow aspirate and biopsy[26, 112] ; however, some oncologists in the United States do not consider bone marrow analysis to be required
The NCCN considers the following essential in the workup of patients diagnosed with CLL[5] :
Physical examination with attention to node-bearing areas, including Waldeyer ring, and measurement of size of liver and spleen
Performance status determination
Assessment of B symptoms
Complete blood count and comprehensive metabolic panel
Treatment
The guidelines are in agreement that patients with early-stage CLL should not be treated with chemotherapy until they become symptomatic or display evidence of rapid progression of disease.[5, 26, 112]
Patients at low risk and intermediate risk (ie, Rai stages 0-II) who have no indications for treatment should be observed. Blood cell counts and clinical examinations should be performed every 3–12 months. Indications for treatment include the following:
Significant disease-related symptoms (eg, fatigue, night sweats, weight loss, fever without infection)
Threatened end-organ function
Progressive bulky disease
Progressive anemia or thrombocytopenia
Progressive lymphocytosis
Therapy recommendations for patients at high risk (ie, Rai stages III-IV) and progressive cytopenia are based on the following:
Patient age
Patient comorbidities
Presence or absence of del(17p)
Presence or absence of TP53 mutations
For first-line treatment of CLL without del(17p) or TP53 mutations, NCCN recommendations (including category 1, which are based on high-level evidence, with uniform NCCN consensus that the intervention is appropriate) are as follows[5] :
Patients < 65 years, without significant comorbidities:
Other recommended regimens: Bendamustine (70 mg/m2 in cycle 1 with escalation to 90 mg/m2 if tolerated) + anti-CD20 monoclonal antibody (not recommended for use in frail patients); chlorambucil + obinutuzumab; obinutuzumab monotherapy; HDMP + rituximab or obinutuzumab (category 2B), ibrutinib + obinutuzumab (category 2B), chlorambucil monotherapy (category 3); rituximab monotherapy (category 3)
Post–first-line maintenance therapy:
Consider lenalidomide for high-risk patients
Preferred second-line and subsequent therapy regimens for CLL patients without del(17p) or TP53 mutations of all ages and comorbidity status are as follows:
Other recommended regimens include alemtuzumab ± rituximab; lenalidomide ± rituximab; HDMP + rituximab; idelalisib ± rituximab; ofatumumab monotherapy; duvelisib monotherapy
Post–first-line maintenance therapy:
Consider lenalidomide for high-risk patients
Post–second-line therapy (ie, for patients with complete or partial response to relapsed/refractory therapy):
Lenalidomide, ofatumumab
The ESMO guidelines recommend that for symptomatic early-stage or advanced-stage CLL that lacks IGHV and TP53 mutations or del(17), patients who are fit should receive treatment with ibrutinib. An alternative, if that agent is contraindicated or unavailable, is chemoimmunotherapy with fludarabine, cyclophosphamide and rituximab (FCR); bendamustine plus rituximab (BR) may be considered in patients older than 65 years. In unfit patients, ESMO recommends venetoclax plus obinutuzumab, ibrutinib, or acalabrutinib; an alternative, if targeted therapies are contraindicated or unavailable is chlorambucil plus obinutuzumab.[112]
In patients with symptomatic early-stage or advanced-stage CLL that is IGHV mutated butdoes not have TP53 mutations or del(17), ESMO guidelines recommend that patients who are fit receive treatment with FCR (or BR, in patients older than 65 years) or ibrutinib. Recommended regimens in unfit patients are as follows:
Venetoclax plus obinutuzumab
Chlorambucil plus obinutuzumab
Ibrutinib or acalabrutinib
For CLL with TP53 mutation or del(17p), ESMO recommendations are as follows:
Ibrutinib or acalabrutinib
Venetoclax with or without obinutuzumab
Idelalisib plus rituximab
For symptomatic relapsed CLL with TP53 mutation or del(17p), ESMO recommendations are as follows:
Ibrutinib or acalabrutinib
Venetoclax plus rituximab
Venetoclax monotherapy
Idelalisib plus rituximab
Consideration of allogeneic hematopoietic stem cell transplantation (HSCT) in fit patients
For symptomatic relapses that occur after remission duration of less than 36 months, ESMO recommendations are as follows:
Ibrutinib or acalabrutinib
Venetoclax plus rituximab
Venetoclax monotherapy
Idelalisib plus rituximab
For relapses that occur after remissions lasting longer than 36 months, ESMO guidelines recommend repeating front-line therapy or changing to one of the following:
Targeted agents are increasingly used in the treatment of chronic lymphocytic leukemia (CLL).
Examples of targeted biologics include:
Bruton tyrosine kinase (BTK) inhibitors
B-Cell lymphoma inhibitors
Monoclonal antibodies (eg, anti-CD20, anti-CD52)
Fludarabine (a purine nucleoside analogue that acts as an antimetabolite) and the alkylating agents bendamustine and chlorambucil are chemotherapy agents that were previously used very commonly in the treatment of CLL. Purine analogues, fludarabine in particular, are very active against CLL. However, recent evidence suggests that the targeted therapies and biologic agents may have less adverse effects and have a significantly higher complete response rate relative to chemotherapy agents, making them more favorable as first-line therapies.
Clinical Context:
Pentostatin inhibits adenosine deaminase, resulting in deoxyadenosine and deoxyadenosine 5+-triphosphate accumulation that may inhibit DNA or RNA synthesis, causing cell death.
Clinical Context:
Chlorambucil is a nitrogen mustard derivative with bifunctional alkylating activity. It forms intrastrand crosslinks, interfering with DNA replication and RNA transcription and translation.
Clinical Context:
A nucleotide analogue of vidarabine, fludarabine is converted to 2-fluoro-ara-A, which enters the cell and is phosphorylated to form active metabolite 2-fluoro-ara-ATP, which inhibits DNA synthesis. Inhibits DNA polymerase and ribonucleotide reductase as well as DNA primase and DNA ligase I.
Clinical Context:
Bruton tyrosine kinase (BTK) inhibitor; acalabrutinib and its active metabolite, ACP-5862, form a covalent bond with a cysteine residue in the BTK active site, leading to inhibition of BTK enzymatic activity
Clinical Context:
Ibrutinib is a Bruton tyrosine kinase (BTK) inhibitor that forms a covalent bond with a cysteine residue in the BTK active site, leading to inhibition of BTK enzymatic activity. BTK is a signaling molecule of the B-cell antigen receptor (BCR) and cytokine receptor pathways. It is indicated for CLL including patients who are treatment-naïve or have been previously treated. It is also indicated for patients who carry a deletion in chromosome 17 (del 17p CLL), which is associated with poor responses to standard treatment.
Clinical Context:
BTK inhibitor that is indicated for chronic lymphocytic leukemia or small lymphocytic lymphoma (CLL/SLL) in adults who have received at ≥2 prior lines of therapy, including a BTK inhibitor and a BCL-2 inhibitor.
Clinical Context:
Small-molecule inhibitor of Bruton tyrosine kinase (BTK), with significant specificity for BTK. It forms a covalent bond with a cysteine residue in the BTK active site, leading to inhibition of BTK activity, preventing cell proliferation. FDA granted accelerated approval granted accelerated approval for zanubrutinib to treat patients with CLL or SLL.
In B-cells, Bruton tyrosine kinase (BTK) signaling results in activation of pathways necessary for B-cell proliferation, trafficking, chemotaxis, and adhesion.
Clinical Context:
Ofatumumab is an anti-CD20 human monoclonal antibody that inhibits B-cell activation in early stages. It is indicated for CLL refractory to fludarabine and alemtuzumab, for previously untreated CLL in combination with chlorambucil, as extended treatment (as a single agent) for patients who are in complete or partial response after at least 2 lines of therapy for recurrent or progressive CLL, and for refractory CLL in combination with fludarabine and cyclophosphamide.
Clinical Context:
Obinutuzumab is a CD20-directed cytolytic antibody, which, upon binding to CD20, mediates B-cell lysis. Mediation may be through engagement of immune effector cells, by directly activating intracellular death-signaling pathways, and/or by activation of the complement cascade. The CD20 antigen is expressed on the surface of pre–B cells and mature B lymphocytes. The immune effector cell mechanisms include antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis. It is indicated for previously untreated CLL in combination with chlorambucil.
Clinical Context:
Alemtuzumab is a humanized monoclonal antibody against CD52, an antigen found on B-cells, T-cells, and almost all CLL cells. It binds to the CD52 receptor of the lymphocytes, which slows the proliferation of leukocytes. Indicated as a single agent for treatment of B-cell chronic lymphocytic leukemia (B-CLL).
Clinical Context:
Rituximab is a humanized murine monoclonal antibody against CD20, an antigen found on B-cells. This agent binds CD20 on lymphocytes and induces apoptosis as well as initiating complement-mediated killing of bound cells. Because CLL B-cells have low levels of CD20 expression, increased doses of rituximab may be necessary. It is available as an IV formulation.
Clinical Context:
The SC product is combined with hyaluronidase human, which increases subcutaneous tissue permeability. It is indicated, in combination with fludarabine and cyclophosphamide (FC), for treatment of adults with previously untreated and previously treated CLL.
Monoclonal antibodies are antibody proteins that are produced from a particular lineage and function to target a particular epitope, or marker, that is usually present on the leukemic cells.
Clinical Context:
Venetoclax is a selective inhibitor of the B-cell lymphoma 2 (Bcl-2) regulator protein, an antiapoptotic protein. Overexpression of Bcl-2 has been demonstrated in CLL cells where it mediates tumor cell survival and has been associated with resistance to chemotherapeutic agents. It is indicated for patients with CLL as monotherapy or in combination with obinutuzumab and/or rituximab.
Overexpression of B-cell lymphoma 2 (Bcl-2) has been demonstrated in CLL cells where it mediates tumor cell survival and has been associated with resistance to chemotherapeutic agents.
Clinical Context:
Selective oral small molecule inhibitor of PI3K-delta and PI3K-gamma. Inhibiting PI3K induces growth inhibition and reduces viability in cell lines derived from malignant B cells and in primary CLL tumor cells. It is indicated for adults with relapsed/refractory chronic lymphocytic leukemia (CLL) or small lymphocytic lymphoma (SLL) after at least 2 prior therapies.
Clinical Context:
Idelalisib is a phosphoinositide 3-kinase (PI3K) delta inhibitor. Idelalisib induces apoptosis and inhibits proliferation in cell lines derived from malignant B cells and in primary tumor cells; also inhibits several cell- signaling pathways, including B cell receptor (BCR) signaling and the CXCR4 and CXCR5 signaling, which are involved in trafficking and homing of B cells to the lymph nodes and bone marrow. It is indicated relapsed chronic lymphocytic leukemia (CLL).
Phosphoinositide 3-kinase (PI3K) delta inhibitors induce apoptosis and inhibit proliferation in cell lines derived from malignant B cells. PI3K delta kinase is expressed in normal and malignant B cells.
What is chronic lymphocytic leukemia (CLL)?What are the signs and symptoms of chronic lymphocytic leukemia (CLL)?How can chronic lymphocytic leukemia (CLL) diagnosed?What are the staging systems for chronic lymphocytic leukemia (CLL)?How is chronic lymphocytic leukemia (CLL) managed?What is the role of clonal B cells in the pathophysiology of chronic lymphocytic leukemia (CLL)?What is the role of genetics in the pathogenesis of chronic lymphocytic leukemia (CLL)?What is the role of proto-oncogene bcl2 in the pathophysiology of chronic lymphocytic leukemia (CLL)?What is the role of miRNA15a and miRNA16-1 in the pathophysiology of chronic lymphocytic leukemia (CLL)?What are high-risk genetic markers in chronic lymphocytic leukemia (CLL)?What are poor prognostic indicators for chronic lymphocytic leukemia (CLL)?What causes chronic lymphocytic leukemia (CLL)?What is the incidence of chronic lymphocytic leukemia (CLL) in the US?What is the global incidence of chronic lymphocytic leukemia (CLL)?Which patient groups are at higher risk for chronic lymphocytic leukemia (CLL)?What is the prognosis of chronic lymphocytic leukemia (CLL)?Which clinical history findings are characteristic of chronic lymphocytic leukemia (CLL)?Which physical findings are characteristic of chronic lymphocytic leukemia (CLL)?How is chronic lymphocytic leukemia (CLL) diagnosed?How is mantel cell lymphoma differentiated from chronic lymphocytic leukemia (CLL)?What are the differential diagnoses for Chronic Lymphocytic Leukemia (CLL)?What is the role of a complete blood count (CBC) with differentials in the diagnosis of chronic lymphocytic leukemia (CLL)?What is the role of peripheral blood smear in the diagnosis of chronic lymphocytic leukemia (CLL)?What is the role of serum quantitative immunoglobulin measurement in the evaluation of chronic lymphocytic leukemia (CLL)?How are prolymphocytic leukemia and splenic lymphoma differentiated from chronic lymphocytic leukemia (CLL)?What is the role of bone marrow aspiration and biopsy in the evaluation of chronic lymphocytic leukemia (CLL)?What is the role of imaging in the evaluation of chronic lymphocytic leukemia (CLL)?Which tests does the NCCN consider informative in the evaluation of chronic lymphocytic leukemia (CLL)?What are potential prognostic markers for chronic lymphocytic leukemia (CLL)?How is bone marrow aspiration and biopsy used in the evaluation of chronic lymphocytic leukemia (CLL)?What are the staging systems for chronic lymphocytic leukemia (CLL)?What is the Rai-Sawitsky staging system for chronic lymphocytic leukemia (CLL)?What is the Binet staging system for chronic lymphocytic leukemia (CLL)?When is chemotherapy indicated in the treatment of chronic lymphocytic leukemia (CLL)?What is included in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of allogeneic stem cell transplantation in the treatment of chronic lymphocytic leukemia (CLL)?What are autoimmune manifestations in chronic lymphocytic leukemia (CLL)?What is the prevalence of autoimmune manifestations in chronic lymphocytic leukemia (CLL)?What is the role of prednisone in the treatment of chronic lymphocytic leukemia (CLL)?What causes hyperviscosity syndrome in chronic lymphocytic leukemia (CLL) cause?What is the role of splenectomy in the treatment of chronic lymphocytic leukemia (CLL)?Which chemotherapy and biologic regimens are used in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of bendamustine in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of alemtuzumab in the treatment of chronic lymphocytic leukemia (CLL)?Which monoclonal antibodies are used in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of ofatumumab (Arzerra) in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of obinutuzumab (Gazyva) in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of duvelisib (Copiktra) in the treatment of chronic lymphocytic leukemia (CLL)?Which monoclonal antibodies are being investigated for the treatment of chronic lymphocytic leukemia (CLL)?What is the role of combination therapies with monoclonal antibodies in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of ibrutinib in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of acalabrutinib in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of idelalisib in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of venetoclax in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of lenalidomide in the treatment of chronic lymphocytic leukemia (CLL)?How is the role of combination venetoclax and obinutuzumab in the treatment of chronic lymphocytic leukemia (CLL)?What is the role of CAR T-cell therapy in the treatment of chronic lymphocytic leukemia (CLL)?How is chronic lymphocytic leukemia (CLL) managed in elderly patients?What are the ESMO diagnostic guidelines for chronic lymphocytic leukemia (CLL)?Which organizations have published guidelines for diagnosing and treating chronic lymphocytic leukemia (CLL)?What are the diagnostic guidelines for chronic lymphocytic leukemia (CLL)?What are National Comprehensive Cancer Network (NCCN) recommendations for further testing when the diagnosis of chronic lymphocytic leukemia (CLL) is not established by flow cytometry?What are the IWCLL diagnostic guidelines for chronic lymphocytic leukemia (CLL)?What testing is recommended prior to initiating treatment for chronic lymphocytic leukemia (CLL)?What are the NCCN recommendations for the workup of chronic lymphocytic leukemia (CLL)?What are the indications for treatment of low or intermediate risk chronic lymphocytic leukemia (CLL)?What is the basis for treatment recommendations for high risk chronic lymphocytic leukemia (CLL)?What are NCCN treatment recommendations for chronic lymphocytic leukemia (CLL) without del(17p) or TP53 mutations?What are NCCN and ESMO treatment recommendations for chronic lymphocytic leukemia (CLL) with del(17p) or TP53 mutations?Which medications are used in the treatment of chronic lymphocytic leukemia (CLL)?Which medications in the drug class Antineoplastic Agents are used in the treatment of Chronic Lymphocytic Leukemia (CLL)?
Mohammad Muhsin Chisti, MD, FACP, Associate Professor of Medicine (Hematology and Oncology), Oakland University William Beaumont School of Medicine; Medical Director of Research, Karmanos Cancer Institute
Disclosure: Nothing to disclose.
Coauthor(s)
Bilal M Ali, BS, MD Candidate, Oakland University William Beaumont School of Medicine
Disclosure: Nothing to disclose.
Emma L Herrman, MD, Resident Physician, Department of Internal Medicine, Beaumont Hospital
Disclosure: Nothing to disclose.
Mariam Aoun, MD, Resident Physician, Department of Internal Medicine, Beaumont Hospital – Royal Oak
Disclosure: Nothing to disclose.
Specialty Editors
Mary L Windle, PharmD, Adjunct Associate Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference
Disclosure: Nothing to disclose.
Chief Editor
Emmanuel C Besa, MD, Professor Emeritus, Department of Medicine, Division of Hematologic Malignancies and Hematopoietic Stem Cell Transplantation, Kimmel Cancer Center, Jefferson Medical College of Thomas Jefferson University
Disclosure: Nothing to disclose.
Additional Contributors
Delong Liu, MD, PhD, Professor of Medicine, Division of Oncology/Hematology, New York Medical College; Chief of Hematology, Phelps Memorial Hospital Center; Director of Non-ablative Allogeneic Stem Cell Transplantation Program, Westchester Medical Center; Editor-in-Chief, Journal of Hematology and Oncology
Disclosure: Nothing to disclose.
Haleem J Rasool, MD, FACP, Chair, Department of Oncology, Mayo Clinic Health System, La Crosse, WI
Disclosure: Nothing to disclose.
Karen Seiter, MD, Professor, Department of Internal Medicine, Division of Oncology/Hematology, New York Medical College
Disclosure: Received honoraria from Novartis for speaking and teaching; Received consulting fee from Novartis for speaking and teaching; Received honoraria from Celgene for speaking and teaching.
Muhammad A Mir, MD, FACP, Assistant Professor of Medicine (Hematology, Blood/Marrow Transplant) Milton S Hershey Medical Center, Pennsylvania State University College of Medicine
Disclosure: Nothing to disclose.
Samir C Patel, MD, Fellow, Department of Hematology and Medical Oncology, Metropolitan Hospital, New York Medical College
Disclosure: Nothing to disclose.
Acknowledgements
Michael Perry, MD, MS, MACP† Former Nellie B Smith Chair of Oncology Emeritus, Former Director, Division of Hematology and Medical Oncology, Former Deputy Director, Ellis Fischel Cancer Center, University of Missouri-Columbia School of Medicine
Clarence Sarkodee-Adoo, MD Consulting Staff, Department of Bone Marrow Transplantation, City of Hope Samaritan BMT Program
Disclosure: Takeda Millenium Honoraria Speaking and teaching
Francisco Talavera, PharmD, PhD Adjunct Assistant Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference
PDQ Adult Treatment Editorial Board. Chronic Lymphocytic Leukemia Treatment (PDQ®): Health Professional Version. PDQ Cancer Information Summaries. Bethesda, MD: National Cancer Institute; June 20, 2023.
[Guideline] National Comprehensive Cancer Network. Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma. NCCN. Available at https://www.nccn.org/professionals/physician_gls/pdf/cll.pdf. Version 1.2023 — August 30, 2022; Accessed: January 12, 2023.
Cancer Facts & Figures 2023. American Cancer Society. Available at https://www.cancer.org/content/dam/cancer-org/research/cancer-facts-and-statistics/annual-cancer-facts-and-figures/2023/2023-cancer-facts-and-figures.pdf. Accessed: January 12, 2023.
Cancer Stat Facts: Leukemia — Chronic Lymphocytic Leukemia (CLL). National Cancer Institute Surveillance, Epidemiology, and End Results Program. Available at https://seer.cancer.gov/statfacts/html/clyl.html. Accessed: January 12, 2023.
Castellino AM. 'Possibility of Cure' for Some Patients With CLL. Medscape Medical News. Available at https://www.medscape.com/viewarticle/918027. September 10, 2019; Accessed: January 12, 2023.
Chustecka Z. Ibrutinib in CLL: indication expanded, benefit confirmed. Medscape Medical News. Available at http://www.medscape.com/viewarticle/828980. July 28, 2014; Accessed: January 12, 2023.
Hoang, T., Cheung, M., Chan, K., et al. The Impact of Drug Interactions on Outcomes in Ibrutinib-Treated Patients with Chronic Lymphocytic Leukemia in Routine Clinical Care: A Population-Based Cohort Study. American Society of Hematology. Available at https://ash.confex.com/ash/2022/webprogram/Paper158768.html. December 12, 2022; Accessed: January 12, 2023.
Project Orbis: FDA approves acalabrutinib for CLL and SLL. U.S. Food & Drug Administration. Available at https://www.fda.gov/drugs/resources-information-approved-drugs/project-orbis-fda-approves-acalabrutinib-cll-and-sll. November 21, 2019; Accessed: January 12, 2023.
Real-World Study Shows Patients Treated with IMBRUVICA® (ibrutinib) Were Less Likely to Initiate a Next-Line Treatment than Patients on Acalabrutinib in First-line Chronic Lymphocytic Leukemia. Johnson & Johnson. December 12, 2022. Available at https://www.jnj.com/real-world-study-shows-patients-treated-with-imbruvica-ibrutinib-were-less-likely-to-initiate-a-next-line-treatment-than-patients-on-acalabrutinib-in-first-line-chronic-lymphocytic-leukemia
McKnight P. FDA Oks Zanubrutinib (Brukinsa) for CLL or SLL. Medscape Medical News. Available at https://www.medscape.com/viewarticle/987252. January 20, 2023; Accessed: January 27, 2023.
Venclexta (venetoclax) [package insert]. North Chicago, IL: AbbVie, Inc. May 2019. Available at
FDA approves venetoclax for CLL and SLL. U.S. Food & Drug Administration. Available at https://www.fda.gov/drugs/resources-information-approved-drugs/fda-approves-venetoclax-cll-and-sll. May 15, 2019; Accessed: September 13, 2019.
FDA approves venetoclax for CLL and SLL. U.S. Food Drug Administration. Available at https://www.fda.gov/drugs/resources-information-approved-drugs/fda-approves-venetoclax-cll-and-sll. May 15, 2019; Accessed: November 7, 2022.
Kater AP, et al; GLOW Investigators. Fixed-Duration Ibrutinib-Venetoclax in Patients with Chronic Lymphocytic Leukemia and Comorbidities. N Engl J Med Evidence. May 13, 2022. 1(7):
Three-Drug Combination Therapy Effective in Patients with High-Risk CLL, Trial Shows. Dana-Farber Cancer Institute. December 10, 2022. Available at https://www.dana-farber.org/newsroom/news-releases/2022/three-drug-combination-therapy-effective-in-patients-with-high-risk-cll--trial-shows/
McCall B. Obinutuzumab Active in Elderly Chronic Lymphocytic Leukemia. Medscape Medical News from the 18th Congress of the European Hematology Association (EHA). Available at http://www.medscape.com/viewarticle/806700. June 21, 2013; Accessed: January 12, 2023.
Nelson R. FDA Grants Full Approval to Ofatumumab (Arzerra) for CLL. Medscape Medical News. Available at http://www.medscape.com/viewarticle/823837. April 17, 2014; Accessed: January 12, 2023.
Robak, T, Warzocha, K, Babu, KG, et al. Health-Related Quality of Life and Patient-Reported Outcomes in Patients Receiving Ofatumumab in Combination with Fludarabine and Cyclophosphamide (FC) Versus FC Alone in the Complement 2 Trial. Blood. 2015. 126(23):5288.
Novartis Withdraws Chronic Leukemia Drug Arzerra From Non-U.S. Markets. FDA News. Available at https://www.fdanews.com/articles/185419-novartis-withdraws-chronic-leukemia-drug-arzerra-from-non-us-markets. January 30, 2018; Accessed: January 12, 2023.
Chustecka Z. Idelalisib (Zydelig) approved for CLL and lymphoma. Medscape Medical News. July 23, 2014.
FDA news release: FDA approves Zydelig for three types of blood cancers. US Food and Drug Administration. July 23, 2014. Available at http://www.fda.gov/NewsEvents/Newsroom/PressAnnouncements/ucm406387.htm
Gribben JG, Jurczak W, Jacobs RW, et al. Umbralisib Plus Ublituximab (U2) Is Superior to Obinutuzumab Plus Chlorambucil (O+Chl) in Patients with Treatment Naïve (TN) and Relapsed/Refractory (R/R) Chronic Lymphocytic Leukemia (CLL): Results from the Phase 3 Unity-CLL Study. Blood. November 5, 2020. 136 suppl 1:37-39.
Ukoniq (umbralisib): Drug Safety Communication - FDA Approval of Lymphoma Medicine is Withdrawn Due to Safety Concerns. U.S. Food & Drug Administration. Available at https://www.fda.gov/safety/medical-product-safety-information/ukoniq-umbralisib-drug-safety-communication-fda-approval-lymphoma-medicine-withdrawn-due-safety. June 1, 2022;
Chustecka Z. Ibrutinib (Imbruvica) Approved for CLL in US. Medscape [serial online]. Available at http://www.medscape.com/viewarticle/820537. February 13, 2014; Accessed: January 12, 2023.
Nelson R. FDA Gives Arzerra Breakthrough Therapy Designation for CLL. Medscape Medical News. Available at http://www.medscape.com/viewarticle/811307. September 19, 2013; Accessed: January 12, 2023.
Smith J. FDA approves ibrutinib plus obinutuzumab for CLL/SLL. MDedge. Available at https://www.mdedge.com/hematology-oncology/article/193367/cll/fda-approves-ibrutinib-plus-obinutuzumab-cll/sll. January 28, 2019; Accessed: November 7, 2022.
Peripheral smear from a patient with chronic lymphocytic leukemia, small lymphocytic variety.
Peripheral smear from a patient with chronic lymphocytic leukemia, large lymphocytic variety. Smudge cells are also observed; smudge cells are the artifacts produced by the lymphocytes damaged during the slide preparation.
Peripheral smear from a patient with chronic lymphocytic leukemia, small lymphocytic variety.
Peripheral smear from a patient with chronic lymphocytic leukemia, large lymphocytic variety. Smudge cells are also observed; smudge cells are the artifacts produced by the lymphocytes damaged during the slide preparation.
CLL-IPI category
Overall Survival at 5 years
Treatment Recommendation
Low risk
93.2%
Do not treat
Intermediate risk
79.3%
Treat only if symptomatic
High risk
63.3%
Treatment indicated; however, can consider no treatment if asymptomatic
Very high risk
23.3%
If treatment needed, do not use chemotherapy; instead, use targeted agent or clinical trial
){kind=link}
){kind=link}